Human memory T cell responses to SARS-CoV E protein.
Identifieur interne : 003E72 ( Main/Exploration ); précédent : 003E71; suivant : 003E73Human memory T cell responses to SARS-CoV E protein.
Auteurs : Hui Peng [République populaire de Chine] ; Li-Tao Yang ; Jian Li ; Zhi-Qiang Lu ; Ling-Yun Wang ; Richard A. Koup ; Robert T. Bailer ; Chang-You WuSource :
- Microbes and infection [ 1286-4579 ] ; 2006.
Descripteurs français
- KwdFr :
- Adulte, Cytométrie en flux (), Déterminants antigéniques des lymphocytes T (immunologie), Femelle, Humains, Interféron gamma (immunologie), Lymphocytes T (immunologie), Lymphocytes T CD4+ (immunologie), Lymphocytes T CD8+ (immunologie), Mâle, Mémoire immunologique, Protéines de l'enveloppe virale (immunologie), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (immunologie), Épitopes immunodominants (immunologie).
- MESH :
- immunologie : Déterminants antigéniques des lymphocytes T, Interféron gamma, Lymphocytes T, Lymphocytes T CD4+, Lymphocytes T CD8+, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère, Virus du SRAS, Épitopes immunodominants.
- virologie : Syndrome respiratoire aigu sévère.
- Adulte, Cytométrie en flux, Femelle, Humains, Mâle, Mémoire immunologique.
English descriptors
- KwdEn :
- Adult, CD4-Positive T-Lymphocytes (immunology), CD8-Positive T-Lymphocytes (immunology), Epitopes, T-Lymphocyte (immunology), Female, Flow Cytometry (methods), Humans, Immunodominant Epitopes (immunology), Immunologic Memory, Interferon-gamma (immunology), Male, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), T-Lymphocytes (immunology), Viral Envelope Proteins (immunology).
- MESH :
- chemical , immunology : Epitopes, T-Lymphocyte, Immunodominant Epitopes, Interferon-gamma, Viral Envelope Proteins.
- immunology : CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes.
- methods : Flow Cytometry.
- virology : Severe Acute Respiratory Syndrome.
- Adult, Female, Humans, Immunologic Memory, Male.
Abstract
E protein is a membrane component of severe acute respiratory syndrome coronavirus (SARS-CoV). Disruption of E protein may reduce viral infectivity. Thus, the SARS-CoV E protein is considered a potential target for the development of antiviral drugs. However, the cellular immune responses to E protein remain unclear in humans. In this study, we found that peripheral blood mononuclear cells (PBMCs) from fully recovered SARS individuals rapidly produced IFN-gamma and IL-2 following stimulation with a pool of 9 peptides overlapping the entire E protein sequence. Analysis of the immune responses by flow cytometry showed that both CD4+ and CD8+T cells were involved in the SARS-CoV E-specific immune responses after stimulation with SARS-CoV E peptides. Moreover, the majority of IFN-gamma+CD4+T cells were central memory cells expressing CD45RO+CCR7+CD62L-; whereas IFN-gamma+CD8+ memory T cells were mostly effector memory cells expressing CD45RO-CCR7-CD62L-. The results of T-cell responses to 9 individual peptides indicated that the E protein contained at least two major T cell epitopes (E2 amino acid [aa] 9-26 and E5-6: aa 33-57) which were important in eliciting cellular immune response to SARS-CoV E protein in humans.
DOI: 10.1016/j.micinf.2006.05.008
PubMed: 16844400
Affiliations:
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Koup</name></author><author><name sortKey="Bailer, Robert T" sort="Bailer, Robert T" uniqKey="Bailer R" first="Robert T" last="Bailer">Robert T. Bailer</name></author><author><name sortKey="Wu, Chang You" sort="Wu, Chang You" uniqKey="Wu C" first="Chang-You" last="Wu">Chang-You Wu</name></author></analytic><series><title level="j">Microbes and infection</title><idno type="ISSN">1286-4579</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>CD4-Positive T-Lymphocytes (immunology)</term><term>CD8-Positive T-Lymphocytes (immunology)</term><term>Epitopes, T-Lymphocyte (immunology)</term><term>Female</term><term>Flow Cytometry (methods)</term><term>Humans</term><term>Immunodominant Epitopes (immunology)</term><term>Immunologic Memory</term><term>Interferon-gamma (immunology)</term><term>Male</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>T-Lymphocytes (immunology)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Cytométrie en flux ()</term><term>Déterminants antigéniques des lymphocytes T (immunologie)</term><term>Femelle</term><term>Humains</term><term>Interféron gamma (immunologie)</term><term>Lymphocytes T (immunologie)</term><term>Lymphocytes T CD4+ (immunologie)</term><term>Lymphocytes T CD8+ (immunologie)</term><term>Mâle</term><term>Mémoire immunologique</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Virus du SRAS (immunologie)</term><term>Épitopes immunodominants (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Epitopes, T-Lymphocyte</term><term>Immunodominant Epitopes</term><term>Interferon-gamma</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Déterminants antigéniques des lymphocytes T</term><term>Interféron gamma</term><term>Lymphocytes T</term><term>Lymphocytes T CD4+</term><term>Lymphocytes T CD8+</term><term>Protéines de l'enveloppe virale</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term><term>Épitopes immunodominants</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CD4-Positive T-Lymphocytes</term><term>CD8-Positive T-Lymphocytes</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Flow Cytometry</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Female</term><term>Humans</term><term>Immunologic Memory</term><term>Male</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Cytométrie en flux</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Mémoire immunologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">E protein is a membrane component of severe acute respiratory syndrome coronavirus (SARS-CoV). Disruption of E protein may reduce viral infectivity. Thus, the SARS-CoV E protein is considered a potential target for the development of antiviral drugs. However, the cellular immune responses to E protein remain unclear in humans. In this study, we found that peripheral blood mononuclear cells (PBMCs) from fully recovered SARS individuals rapidly produced IFN-gamma and IL-2 following stimulation with a pool of 9 peptides overlapping the entire E protein sequence. Analysis of the immune responses by flow cytometry showed that both CD4+ and CD8+T cells were involved in the SARS-CoV E-specific immune responses after stimulation with SARS-CoV E peptides. Moreover, the majority of IFN-gamma+CD4+T cells were central memory cells expressing CD45RO+CCR7+CD62L-; whereas IFN-gamma+CD8+ memory T cells were mostly effector memory cells expressing CD45RO-CCR7-CD62L-. The results of T-cell responses to 9 individual peptides indicated that the E protein contained at least two major T cell epitopes (E2 amino acid [aa] 9-26 and E5-6: aa 33-57) which were important in eliciting cellular immune response to SARS-CoV E protein in humans.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><noCountry><name sortKey="Bailer, Robert T" sort="Bailer, Robert T" uniqKey="Bailer R" first="Robert T" last="Bailer">Robert T. Bailer</name><name sortKey="Koup, Richard A" sort="Koup, Richard A" uniqKey="Koup R" first="Richard A" last="Koup">Richard A. Koup</name><name sortKey="Li, Jian" sort="Li, Jian" uniqKey="Li J" first="Jian" last="Li">Jian Li</name><name sortKey="Lu, Zhi Qiang" sort="Lu, Zhi Qiang" uniqKey="Lu Z" first="Zhi-Qiang" last="Lu">Zhi-Qiang Lu</name><name sortKey="Wang, Ling Yun" sort="Wang, Ling Yun" uniqKey="Wang L" first="Ling-Yun" last="Wang">Ling-Yun Wang</name><name sortKey="Wu, Chang You" sort="Wu, Chang You" uniqKey="Wu C" first="Chang-You" last="Wu">Chang-You Wu</name><name sortKey="Yang, Li Tao" sort="Yang, Li Tao" uniqKey="Yang L" first="Li-Tao" last="Yang">Li-Tao Yang</name></noCountry><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Peng, Hui" sort="Peng, Hui" uniqKey="Peng H" first="Hui" last="Peng">Hui Peng</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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